Fig. 6. Role of Sesn2 in 2-DG-induced apoptosis inhuman culture cells. (A-H) Cells treated with or without 2-DG (10 mM, 20 mM, and 40 mM) and combined with overexpression or knockdown of Sesn2 were subjected to western blot analysis to analyse the expression of cleaved caspase 3, cleaved caspase9, and Bcl- 2. NAPDH expression was also analysed as an endogenous control. Quantitative analysis of the western blot data showing the relative protein expression levels. (I-J) Apoptosis analyzed by flow cytometry with Annexin V–PI double staining. The data are presented as the mean ± SD from three independent replicate experiments. *P<0.05 vs. control.